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Valiant Co Ltd sterile glass beads
Sterile Glass Beads, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+glass+beads/Roll+%26+Grow+plating+beads/pmc12518797-236-19-36
Average 96 stars, based on 760 article reviews
sterile glass beads - by Bioz Stars, 2026-09
96/100 stars

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Sterility:

Article Title: Evolution of gut microbiota across honeybee species revealed by comparative metagenomics.
Article Snippet: .. Then, the hindgut tissue was transferred to a bead-beating tube containing 478 μL of sterile PBS and 2.0mm sterile glass beads and subjected to beadbeating for 30 s at 6m/s in FastPrep-24 5 G (MP Biomedicals). .. Next, 0.1mm zirconia beads along with 250 μL of 2x CTAB, 2 μL of β-mercaptoethanol and 20 μL of proteinase K (20mg/mL) was added and bead-beating was carried out for 30 s at 6m/s in FastPrep-24 5G (MP Biomedicals).

Article Title: Detection of Esca‐Associated Fungi in Grapevine Trunks Using Loop‐mediated Isothermal Amplification (LAMP) Assays
Article Snippet: .. Conventional DNA extraction from wood followed the protocol by Inglis et al. (2018) with minor modifications: 150 mg of wood shavings were transferred into sterile 2 mL Eppendorf tubes and treated in 1 mL of sorbitol wash buffer (100 mM Tris–HCl pH 8.0, 0.35 M sorbitol, 5 mM EDTA pH 8.0, 1% [w/v] polyvinylpyrrolidone MW 40,000) in a Fastprep® homogenizer for 45 s at 5 m/s after adding 0.3 g of sterile glass beads (1.25–1.65 mm diameter) as well as one sterile 6.35 mm ceramic sphere (MP Biomedicals, Irvine, CA, USA) per tube. ..

Article Title: The gut microbiota affects the social network of honeybees.
Article Snippet: .. To prepare the inoculum for the CL treatment, we randomly collected five nurse bees from each of three hives and bead-beat their guts in 1 ml of 1× PBS with 0.75–1 mm sterile glass beads using a FastPrep-24 5G homogenizer (MP Biomedicals) at 6 m s−1 for 45 s. We pooled the five gut homogenates by hive of origin and plated a serial dilution of these pools from 10−3–10−12 onto BHIA, CBA + blood and MRSA + 0.1% l-cys + 2% fructose using the drop method (10 μl droplets). ..

Article Title: Microplastics exhibit accumulation and horizontal transfer of antibiotic resistance genes.
Article Snippet: Although the fates of microplastics (0.1–5 mm) in marine environments and freshwater are increasingly studied, little is known about their vector effect in wastewater treatment plants (WWTPs).. Previous studies have evaluated the accumulation of antibiotic resistance genes (ARGs) on microplastics, but there is no direct evidence for the selection and horizontal transfer of ARGs on different microplastics in WWTPs.. Here, we show biofilm formation as well as bacterial community and ARGs in these biofilms grown on four kinds of microplastics via incubation in the aerobic and anaerobic tanks of a WWTP.

Article Title: Evolution of gut microbiota across honeybee species revealed by comparative metagenomics
Article Snippet: .. Then, the hindgut tissue was transferred to a bead-beating tube containing 478 μL of sterile PBS and 2.0 mm sterile glass beads and subjected to bead-beating for 30 s at 6 m/s in FastPrep-24 5 G (MP Biomedicals). .. Next, 0.1 mm zirconia beads along with 250 μL of 2x CTAB, 2 μL of β-mercaptoethanol and 20 μL of proteinase K (20 mg/mL) was added and bead-beating was carried out for 30 s at 6 m/s in FastPrep-24 5 G (MP Biomedicals).

Article Title: Commensal Fungus Candida albicans Maintains a Long-Term Mutualistic Relationship with the Host To Modulate Gut Microbiota and Metabolism
Article Snippet: .. Briefly, about 200 mg of stool sample was resuspended in Tris-EDTA buffer (pH 8.0) and homogenized using sterile glass beads (2.5 mm), and the suspension was collected after centrifugation followed by enzymatic lysis using a cocktail of lysozyme (10 mg/mL) (MFCD00131557; Mp Biomedicals India Pvt., Ltd.), Zymolyase (20 mg/mL) (08320921; Mp Biomedicals India, Pvt., Ltd.), and lyticase (20 mg/mL) (37340-57-1; Sigma-Aldrich) at 37°C overnight. ..

Article Title: Symbiont loss and gain, rather than co-diversification shapes honeybee gut microbiota diversity and function
Article Snippet: .. Then, the tissue was transferred to a bead-beating tube containing 478 μL of sterile PBS and 2.0 mm sterile glass beads and subjected to bead-beating for 30s at 6 m/s in FastPrep-24 5G (MP Biomedicals). .. Next, 0.1 mm zirconia beads along with 250 μL of 2x CTAB, 2 μL of β-mercaptoethanol and 20 μL of proteinase K (20 mg/mL) was added and bead-beating was carried out for 30s at 6 m/s in FastPrep-24 5G (MP Biomedicals).

Article Title: The gut microbiota affects the social network of honeybees.
Article Snippet: .. On the afternoon of the second day, we dissected one newly emerged bee per box, homogenized their hindguts in 1 ml of 1× PBS with 0.75– 1 mm sterile glass beads using a Fast-Prep24 5G homogenizer (MP Biomedicals) at 6 m s−1 for 45 s. We then plated these homogenates on BHIA, CBA + blood and MRSA + 0.1% l-cys + 2% fructose growth media with the drop method and cultured them overnight in anaerobic and microaerophilic conditions. ..

DNA Extraction:

Article Title: Detection of Esca‐Associated Fungi in Grapevine Trunks Using Loop‐mediated Isothermal Amplification (LAMP) Assays
Article Snippet: .. Conventional DNA extraction from wood followed the protocol by Inglis et al. (2018) with minor modifications: 150 mg of wood shavings were transferred into sterile 2 mL Eppendorf tubes and treated in 1 mL of sorbitol wash buffer (100 mM Tris–HCl pH 8.0, 0.35 M sorbitol, 5 mM EDTA pH 8.0, 1% [w/v] polyvinylpyrrolidone MW 40,000) in a Fastprep® homogenizer for 45 s at 5 m/s after adding 0.3 g of sterile glass beads (1.25–1.65 mm diameter) as well as one sterile 6.35 mm ceramic sphere (MP Biomedicals, Irvine, CA, USA) per tube. ..

Serial Dilution:

Article Title: The gut microbiota affects the social network of honeybees.
Article Snippet: .. To prepare the inoculum for the CL treatment, we randomly collected five nurse bees from each of three hives and bead-beat their guts in 1 ml of 1× PBS with 0.75–1 mm sterile glass beads using a FastPrep-24 5G homogenizer (MP Biomedicals) at 6 m s−1 for 45 s. We pooled the five gut homogenates by hive of origin and plated a serial dilution of these pools from 10−3–10−12 onto BHIA, CBA + blood and MRSA + 0.1% l-cys + 2% fructose using the drop method (10 μl droplets). ..

Crocin Bleaching Assay:

Article Title: The gut microbiota affects the social network of honeybees.
Article Snippet: .. To prepare the inoculum for the CL treatment, we randomly collected five nurse bees from each of three hives and bead-beat their guts in 1 ml of 1× PBS with 0.75–1 mm sterile glass beads using a FastPrep-24 5G homogenizer (MP Biomedicals) at 6 m s−1 for 45 s. We pooled the five gut homogenates by hive of origin and plated a serial dilution of these pools from 10−3–10−12 onto BHIA, CBA + blood and MRSA + 0.1% l-cys + 2% fructose using the drop method (10 μl droplets). ..

Article Title: The gut microbiota affects the social network of honeybees.
Article Snippet: .. On the afternoon of the second day, we dissected one newly emerged bee per box, homogenized their hindguts in 1 ml of 1× PBS with 0.75– 1 mm sterile glass beads using a Fast-Prep24 5G homogenizer (MP Biomedicals) at 6 m s−1 for 45 s. We then plated these homogenates on BHIA, CBA + blood and MRSA + 0.1% l-cys + 2% fructose growth media with the drop method and cultured them overnight in anaerobic and microaerophilic conditions. ..

Suspension:

Article Title: Commensal Fungus Candida albicans Maintains a Long-Term Mutualistic Relationship with the Host To Modulate Gut Microbiota and Metabolism
Article Snippet: .. Briefly, about 200 mg of stool sample was resuspended in Tris-EDTA buffer (pH 8.0) and homogenized using sterile glass beads (2.5 mm), and the suspension was collected after centrifugation followed by enzymatic lysis using a cocktail of lysozyme (10 mg/mL) (MFCD00131557; Mp Biomedicals India Pvt., Ltd.), Zymolyase (20 mg/mL) (08320921; Mp Biomedicals India, Pvt., Ltd.), and lyticase (20 mg/mL) (37340-57-1; Sigma-Aldrich) at 37°C overnight. ..

Centrifugation:

Article Title: Commensal Fungus Candida albicans Maintains a Long-Term Mutualistic Relationship with the Host To Modulate Gut Microbiota and Metabolism
Article Snippet: .. Briefly, about 200 mg of stool sample was resuspended in Tris-EDTA buffer (pH 8.0) and homogenized using sterile glass beads (2.5 mm), and the suspension was collected after centrifugation followed by enzymatic lysis using a cocktail of lysozyme (10 mg/mL) (MFCD00131557; Mp Biomedicals India Pvt., Ltd.), Zymolyase (20 mg/mL) (08320921; Mp Biomedicals India, Pvt., Ltd.), and lyticase (20 mg/mL) (37340-57-1; Sigma-Aldrich) at 37°C overnight. ..

Lysis:

Article Title: Commensal Fungus Candida albicans Maintains a Long-Term Mutualistic Relationship with the Host To Modulate Gut Microbiota and Metabolism
Article Snippet: .. Briefly, about 200 mg of stool sample was resuspended in Tris-EDTA buffer (pH 8.0) and homogenized using sterile glass beads (2.5 mm), and the suspension was collected after centrifugation followed by enzymatic lysis using a cocktail of lysozyme (10 mg/mL) (MFCD00131557; Mp Biomedicals India Pvt., Ltd.), Zymolyase (20 mg/mL) (08320921; Mp Biomedicals India, Pvt., Ltd.), and lyticase (20 mg/mL) (37340-57-1; Sigma-Aldrich) at 37°C overnight. ..

Cell Culture:

Article Title: The gut microbiota affects the social network of honeybees.
Article Snippet: .. On the afternoon of the second day, we dissected one newly emerged bee per box, homogenized their hindguts in 1 ml of 1× PBS with 0.75– 1 mm sterile glass beads using a Fast-Prep24 5G homogenizer (MP Biomedicals) at 6 m s−1 for 45 s. We then plated these homogenates on BHIA, CBA + blood and MRSA + 0.1% l-cys + 2% fructose growth media with the drop method and cultured them overnight in anaerobic and microaerophilic conditions. ..



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